Journal: Drug Design, Development and Therapy
Article Title: β 3 -Adrenoceptor activation upregulates apolipoprotein A-I expression in HepG2 cells, which might further promote cholesterol efflux from macrophage foam cells
doi: 10.2147/DDDT.S130088
Figure Lengend Snippet: β 3 -AR activation upregulates ApoA-I protein expression in the supernatant of the HepG2 cell. Notes: ( A – D ) ELISA analysis of ApoA-I, ApoA-II, ApoB, and β 3 -AR performed in the medium of HepG2 cell supernatant. Control: HepG2 cells with dimethyl sulfoxide (DMSO) treatment; BRL37344: HepG2 cells with 24 h 10 −6 mol/L BRL37344 treatment; SR59230A: HepG2 cells with 10 −6 mol/L SR59230A treatment. Statistically significant differences are indicated (compared with control group, * P <0.01; ** P <0.05; compared with BRL37344 group, § P <0.05). Abbreviations: Apo, apolipoprotein; β 3 -AR, β3-adrenoceptor; ELISA, enzyme-linked immunosorbent assay.
Article Snippet: ELISA kits for ApoA-I, ApoA-II, ApoB, and β 3 -AR, as well as the BCA protein assay kit, were purchased from Promega (Madison, WI, USA).
Techniques: Activation Assay, Expressing, Enzyme-linked Immunosorbent Assay